Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13087/715
Title: A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis
Authors: Dolak, İbrahim
Canpolat, Gurbet
Onat, Ruken
Keçili, Rüstem
Baysal, Zübeyde
Ziyadanoğulları, Berrin
Say, Rıdvan
Keywords: Molecularly imprinted cryogels
Lanthanide chelate
Hemoglobin
Selective separation
Thalassemia
Issue Date: 2020
Publisher: Elsevier Sci Ltd
Abstract: Purification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.
URI: https://doi.org/10.1016/j.procbio.2019.12.011
https://hdl.handle.net/20.500.13087/715
ISSN: 1359-5113
1873-3298
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Çevre Mühendisliği Bölümü Koleksiyonu

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