Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13087/3211
Title: Screening the immunotoxicity of different food preservative agents on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae) larvae
Authors: Erbaş Duman, Emine
Gwokyalya, Rehemah
Altuntaş, Hülya
Kutrup, Bilal
Keywords: Galleria mellonella
model organism
immunotoxicity
food preservatives
hemocytes
In-Vivo Model
Sodium Benzoate
Hemocytes
Insect
Infection
Host
Combinations
Azadirachtin
Caspofungin
Responses
Issue Date: 2022
Publisher: Taylor & Francis Ltd
Abstract: Immunotoxic effects of sodium benzoate (SB, E211), sodium nitrate (SNa, E251), and sodium nitrite (SNi, E250), a few of the most common food preservatives, on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae) larvae were investigated in this study. The last instar larvae were used for all experimental analyses. For this purpose, median lethal doses of SB, SNa, and SNi were applied to the larvae by the force-feeding method. We found that force-feeding G. mellonella larvae with SB, SNa, and SNi significantly reduced the larval total hemocyte counts, prohemocyte, and granulocyte ratios but increased plasmatocyte, spherulocyte, and oenocyte ratios, as well as the hemocyte mitotic indices and micronucleus frequency. The spreading ability of hemocytes and hemocyte-mediated immune responses were lower in the SB, SNa-, and SNi-treated larval groups compared to controls. Apoptotic indices were higher in all larval groups treated with food preservatives, but increments in necrotic indices were only significantly higher in SNi-treated larvae compared to controls. Our research shows that SB, SNa, and SNi have immunotoxic and cytotoxic potential on G. mellonella larvae. Thus, we suggest that G. mellonella larvae can be used as preliminary in vivo models to screen the immunotoxic effects of food preservative agents.
URI: https://doi.org/10.1080/01480545.2022.2091589
https://hdl.handle.net/20.500.13087/3211
ISSN: 0148-0545
1525-6014
Appears in Collections:Biyoloji Bölümü Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu

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