Determination of lipase activities of actinomycetes isolates from soil

Abstract

In this study 156 actinomycetes isolates which were isolated from different soil samples were used. Isolates were inoculated proper medium and incubated at 28°C for 7-10 days. In order to investigate the lipolytic activities of the isolates in the culture medium, the transparent zone formed by isolates in the prepared lipase medium was measured with a ruler. 53 of them (34 %) 10 mm and less, 99 of them (63 %) 20 mm and less, 4 of them (3 %) more than 20 mm transparent zone produced. The isolate which showed the highest lipase activity molecular identification has been made which based on 16S rRNA gene sequence. The 16S rRNA sequence information obtained from the isolate was recorded to GenBank with the access number KY172825. As a result of the study has been made on the NCBI database by using BLAST program, it was observed that, isolate showed 97% similarity ‘uncultured actinobacterium clone AS42 16S ribosomal RNA gene’ which’s access number on the database is EU283368.1.