Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13087/3197
Title: Antitumor Effects of Turmeric on OVCAR-3 Ovarian Cancer Cell Lines
Authors: Gürer, Hulusi Göktuğ
Gürsoy, Özlem Özgur
Eren, Ceren Yıldız
Sezer, Canan Vejselova
Keywords: Turmeric
OVCAR-3
anti-cancer effects
MTT
Annexin-V
migration assay
Colorimetric Assay
Expression
Growth
Curcumin
Issue Date: 2022
Publisher: Bentham Science Publ Ltd
Abstract: Introduction: Ovarian cancer is the deadliest gynecological malignancy, usually not detected until the late stages. In vitro cell culture is a method used to study the behavior of cells in a controlled environment. Turmeric has attracted the attention of scientists due to its anticancer potential. Methods: OVCAR-3 cells were cultured in RPMI medium with 100 units/mL-100 mu g/mL of penicillin-streptomycin and 10% foetal bovine serum in a CO2 incubator. Turmeric extract was diluted in DMSO. Different concentrations of turmeric extract were prepared. Annexin-V staining was performed to test the translocation of phosphatidylserine to the outer side of the cell membrane as a clear indicator of apoptosis. Results: Turmeric extract significantly reduced the viability of OVCAR-3 cells both within 24 and 48 hours of exposure. OVCAR-3 cells were treated with IC50 concentration of turmeric extract for 24 hours. 82.60% of cells were viable. The percentages of the dead, early apoptotic, and late apoptotic cells were detected to be 0.80%, 9.70%, and 6.90%, respectively. Untreated OVCAR-3 cells had migration ability. OVCAR-3 cells exposed to an IC50 concentration of turmeric extract for 24 hours did not close the scratch area. Conclusion: In this research, anticancer effects of turmeric have been demonstrated by different analysis methods.
URI: https://doi.org/10.2174/1871520622666220426103332
https://hdl.handle.net/20.500.13087/3197
ISSN: 1871-5206
1875-5992
Appears in Collections:Biyoloji Bölümü Koleksiyonu
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Scopus İndeksli Yayınlar Koleksiyonu
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