DETERMINATION OF THE GENETIC VARIATION AMONG SOME RICE (ORYZA SATIVA L.) CULTIVARS USING ISSR TECHNIQUE

Abstract

In this study, the genetic diversity levels of 28 different rice varieties developed in Turkey were determined by using ISSR markers. Twenty different ISSR primers amplified 268 bands of which 217 were polymorphic. Band number for each primer ranged from 5-22 with an average number of 13.4. Polymorphism percentages varied between 40.00% and 94.12% with an average of 80.97%. Polymorphism Information Content (PIC) values were between 0.274 and 0.374 with an average value of 0.347. Similarity values indicated that the most similar varieties were Efe and Hamzadere (0.856) and the least similar varieties were Demir and Bafra Yildizi (0.395). UPGMA analysis grouped 28 rice varieties into two main clusters and showed that genotypes obtained from same institute tend to cluster together. This implies the possibility of natural cross pollination between rice varieties grown in close fields. Our results indicated that ISSR technique is effective to study genetic variation in Turkish rice cultivars. Data obtained from this study could be used in the rice improvement programs.