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|Title:||Antibacterial and antibiotic-modifying activities of fractions and compounds from Albizia adianthifolia against MDR Gram-negative enteric bacteria||Authors:||Tchinda, Cedric Fossi
Simo, Ingrid Konga
Voukeng, Igor K.
Nganou, Blaise Kemajou
Antibiotic modifying activity
|Issue Date:||2019||Publisher:||Bmc||Abstract:||BackgroundAlbizia adianthifolia (Schum.) is medicinally used in Cameroon to manage bronchitis and skin diseases. Our previous study documented the antibacterial potential of its roots' methanol extract. In this study, methanol roots extract was subjected to chromatography techniques and fractions (AARa and AARb), sub-fractions (AARa1-4, AARb1-2 and AARb11-14) together with isolated phytochemicals were assessed for their antimicrobial as well as their antibiotic-potentiating effects towards Gram-negative multidrug resistant (MDR) bacteria.MethodsThe antibacterial activities of the samples (determination of Minimal Inhibitory << MIC >> and Minimal Bactericidal Concentration << MBC >>) were determined by the modified rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay, as well as those of antibiotics in association with the compounds. Column chromatography was applied to isolate phytochemicals from roots extract and their chemical structures were determined using spectroscopic techniques.ResultsThe phytochemicals isolated were stearic acid (1), a mixture (1:1) of stigmasterol and -sitosterol (2+3), -sitosterol 3-O--(D)-glucopyranoside (4), palmatin (5), homomangiferin (6) and mangiferin (7). Fraction AARa exhibited selective inhibitory effects whilst all tested bacteria were inhibited by AARb in MIC ranges of 8 to 1024g/mL. Sub-fractions AARb1-2 had MIC values between 8g/mL and 1024g/mL on all tested bacteria. Phytochemicals 4, 2+3 and 7 inhibited the growth of 54.54% (6/11), 45.45% (5/11) and 27.27% (3/11) tested bacterial strains, respectively. When tested with an efflux pumps inhibitor (Phenylalanine-Arginine--Naphthylamide or PAN), the inhibitory effects of compounds 2+3 and 4 increased towards all the tested bacteria. In association with erythromycin (ERY), streptomycin (STR) and tetracycline (TET), compounds 2+3 and 4 had the most significant synergistic activity on the seven selected bacteria.ConclusionThe present study provides information on the possible use of Albizia adianthifolia and its constituents in the control of Gram-negative infections including MDR phenotypes.||URI:||https://doi.org/10.1186/s12906-019-2537-1
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