ANTIBIOTIC RESISTANT BACTERIA PROFILE AND MICROBIAL COMMUNITY STRUCTURE OF TROUT FARMING PONDS IN BILECIK, TURKEY

Abstract

In the study, samples were taken from the three different trout farming ponds of Bilecik, Turkey. Both culture-dependent and culture-independent methods were used to determine microbial load, diversity and antibiotic resistant bacteria in these ponds. Counts of mesophilic and coliform bacteria groups in the samples were determined. In addition, in order to determine antibiotic resistant bacterial groups, isolation was performed using a medium containing seperately sulfamethacin, sulfamerazine, erythromycin and tetracycline and the isolates were identified by performing rDNA sequence analysis. Within the scope of culture independent studies, total DNA extraction was performed from the water samples taken from the ponds and a next-generation sequencing method was applied to the samples. As a result of experimental data, antibiotic resistant isolates were determined as Pantoea sp., Pseudomonas sp., Aeromonas media, Hafnia alvei, Aeromonas rivipollensis, Pseudomonas protegens, Staphylococcus sp., Kluyvera intermedia, Bacillus pumilus, Aeromonas salmonicida, Bacillus zhangzhouensis and these isolates were found to be resistant to all 4 antibiotics. When analyzing illumina DNA sequencing data, Most of the reads were assigned to the class Gammaproteobacteria and Betaproteobacteria and order Enterobacteriales and Burkholderiales.