Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.13087/1013
Title: Characteristics of apoptosis induction in human breast cancer cells treated with a ceramidase inhibitor
Authors: İzgördü, Hüseyin
Sezer, Canan Vejselova
Çömlekçi, Emre
Kutlu, Hatice Mehtap
Keywords: MCF-7
d-erythro-MAPP
Nanoparticle
Cancer
Sphingolipid
Issue Date: 2020
Publisher: Springer
Abstract: Cancer is a complex disease with high mortality rates. Breast cancer is one of the most fatal diseases both for men and woman. Despite the positive developments on cancer treatment, a successful treatment agent/method has not been developed, yet. Recently, cancer research has been involved in sphingolipid metabolism. The key molecule here is ceramide. Ceramides mediate growth suppress, apoptosis and aging regulation. Ceramidases metabolize ceramide and decrease its level in cells and cause escape the death. Inhibition of ceramidases as new targets for cancer treatment is shown in the literature. Herein, we found that d-erythro-MAPP and its nanoparticle formulation, reduce the viability of MCF-7 cells in a dose-dependent manner with IC50 value of 4.4 mu M, and 15.6 mu M, respectively. Confocal and transmission electron microscopy results revealed apoptotic morphological and ultrastructural changes for both agents. Apoptosis and cell cycle arrest were supported by annexin-V, mitochondrial membrane potential changings and cell cycle analysis, respectively.
URI: https://doi.org/10.1007/s10616-020-00436-1
https://hdl.handle.net/20.500.13087/1013
ISSN: 0920-9069
1573-0778
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu

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